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Ascertainment Biases in SNP Chips Affect Measures of Population Divergence

Filed under Diagnostics, Genotyping by on October 14, 2010 at 7:00 pm no comments
SNP genotyping
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In this study, we investigate the effect of the ascertainment bias on inferences regarding genetic differentiation among populations in one of the common genome-wide genotyping platforms. We generate SNP genotyping data for individuals that previously have been subject to partial genome-wide Sanger sequencing and compare inferences based on genotyping data to inferences based on direct sequencing. In addition, we also analyze publicly available genome-wide data. We demonstrate that the ascertainment biases will distort measures of human diversity and possibly change conclusions drawn from these measures in some times unexpected ways. We also show that details of the genotyping calling algorithms can have a surprisingly large effect on population genetic inferences. We not only present a co...
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Automated Benchtop System Prepares Samples for the Roche GS FLX DNA Sequencer

Filed under Core facilities, Diagnostics by on October 10, 2010 at 9:00 pm no comments
Animation of the structure of a section of DNA...
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The SPRIworks Fragment Library System II from Beckman Coulter Genomics is the second release in the SPRIworks product line and automates the preparation of fragment libraries for the Roche GS FLX* DNA Sequencer. It can also prepare up to 10 DNA libraries in 3.5 hours with high reproducibility and consistency.
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Human Genome Assembly and Analysis on a Desktop Computer Using DNASTAR’s Prototype Pipeline

Filed under Bioinformatics, Genotyping by on October 7, 2010 at 9:00 pm no comments
SAN FRANCISCO - JULY 15: The Intel logo is se...
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This application note demonstrates an innovative approach to next-generation sequence assembly and analysis pipeline that will run on a desktop computer and produce a human genome sequence in a reasonable time frame.
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Large-scale SNP genotyping in crosses between outbred lines: how useful is it?

Filed under Breeding, Genotyping by on October 2, 2010 at 11:03 pm no comments
European population substructure
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Large-scale SNP genotyping in crosses between outbred lines: how useful is it? Heredity. 2010 Aug;105(2):173-82 Authors: Ledur MC, Navarro N, Pérez-Enciso M Although genome-wide association (GWA) studies are not worth the effort in crosses between inbred lines, many crosses are actually made up of divergent yet outbred populations. Despite its relevance, however, this experimental setting has not been studied at a time when SNP microarrays are available in many species. To assess whether GWA can be useful in this setting, we performed combined coalescence--gene dropping simulations. We studied the influence of marker density, QTL effect and QTL allele frequency on power, false discovery rate (FDR) and accuracy. Our results suggest that GWA in outbred F(2) crosses is useful, especially in large populations. Under these circumstances, accuracy increased and FDR decreased as compared with classical linkage analysis. However, current SNP densities (in the order of 30-60 K SNPs/genome or equivalent to 10-20 SNPs per cM) may not be much better than linkage analysis and higher SNP densities may be required. SNP ascertainment had an important effect; the best option was to select SNPs as uniformly as possible without setting any restriction on allele frequency. Using only SNPs with fixed alternative alleles in each breed controlled false positive rate but was not useful to detect variability within lines. Finally, the most significant SNP was not necessarily the closest to the causal SNP, although the closest SNPs were usually above the significance threshold; thus, it is prudent to follow-up significant signals located in regions of interest even if they do not correspond to absolute maxima. PMID: 19844266 [PubMed - in process]
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