A Taqman assay for high-throughput genotyping of the multiple sclerosis-associated HLA-DRB1*1501 allele.

Tissue Antigens. 2008 Oct;72(4):401-3

Authors: Goris A, Walton A, Ban M, Dubois B, Compston A, Sawcer S

The human leukocyte antigen (HLA)-DRB1*1501 allele has long been established as the main genetic risk factor for multiple sclerosis (MS), and it therefore follows that stratification of study populations for this allele could aid in the identification of novel susceptibility genes and/or in establishing interactions. To this end, we have developed a simple Taqman-based assay allowing cost-efficient medium-throughput HLA-DRB1*1501 genotyping. We have validated this assay in 444 trio families with MS and 1066 individuals from the UK 1958 birth cohort (3908 independent chromosomes). In this validation cohort, the correlation coefficient (r(2)) between rs3135388*A and HLA-DRB1*1501 was >0.94. Subsequently, applying the assay to a group of MS patients and controls from Belgium confirmed the association of HLA-DRB1*1501 and MS in this population (P = 5 x 10(-21)).

PMID: 18647361 [PubMed - indexed for MEDLINE]

TaqMan-based real-time PCR for genotyping common polymorphisms of haptoglobin (HP1 and HP2).

Clin Chem. 2008 Nov;54(11):1908-13

Authors: Soejima M, Koda Y

The haptoglobin gene (HP) has 2 common codominant alleles (HP(1) and HP(2)) that account for 3 phenotypes. HP(2) is generated by a 1.7-kb intragenic duplication of HP(1).

PMID: 18787013 [PubMed - indexed for MEDLINE]

A real-time Taqman method for hepatitis C virus genotyping and methods for further subtyping of isolates.

Methods Mol Biol. 2009;510:55-71

Authors: Rolfe KJ, Wreghitt TG, Alexander GJ, Curran MD

The HCV genome is highly heterogeneous; more and more genotypes, each with several distinct subtypes, are being identified around the world. Knowledge of genotype is important for planning of treatment regimes, whereas subtype identification is useful in epidemiological studies and outbreak investigation. We describe HCV genotyping and subtyping assays, based on real-time PCR, that are sensitive, specific, and reliable. These assays provide fast, accurate, and convenient methods for HCV genotyping/subtyping to support clinical practice.

PMID: 19009253 [PubMed - indexed for MEDLINE]

[Genotyping of AKAP10 gene 2073A/G single nucleotide polymorphism by TaqMan probe real-time PCR].

Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Mar;40(2):275-8

Authors: Wang MJ, Zhou ZG, Wang L, Li Y, Zhang P, Zhang Y, Cui CF, Zhou B

To detect AKAP10 gene 2073A/G single nucleotide polymorphism (SNP) genotyping by TaqMan probe real-time PCR.

PMID: 19462906 [PubMed - indexed for MEDLINE]